His main research activity aims at the biophysical study of biological molecules and macromolecules both in situ and in vitro utilizing almost not perturbative instrumentation and nanostructured model systems. So far, his research deals with the design, realization and utilization of biophysical instrumentation as conventional, confocal and multi-photon fluorescence microscopy, SHG imaging microscopy, FLIM, single molecule imaging, STED nanoscopy. In particular, he developed an original setup for SW 2PE-STED (single wavelength two-photon excitation stimulated emission depletion) super-resolution microscopy and another for SHG (second harmonic generation) combined with 2PEF (2-photon excitation fluorescence) microscopy. He recently applied STED nanoscopy technique to the quantitative study of single molecule diffusion in living cells. Moreover, he is in charge of the scientific management of the Nikon Imaging Center and he is responsible for the partnership with the R&D of Nikon Instruments Japan.
Telefono
+39 010 2897 613
Research center
CHT@Erzelli
Biografia
Top Publications
2021
Storti B., Quaranta P., Di Primio C., Clementi N., Mancini N., Criscuolo E., Spezia P.G., Carnicelli V., Lottini G., Paolini E., Freer G., Lai M., Costa M., Beltram F., Diaspro A., Pistello M., Zucchi R., Bianchini P., Signore G., Bizzarri R.
A spatial multi-scale fluorescence microscopy toolbox discloses entry checkpoints of SARS-CoV-2 variants in Vero E6 cells
Computational and Structural Biotechnology Journal, vol. 19, pp. 6140-6156
Article
Journal
2021
Jadavi S., Bianchini P., Cavalleri O., Dante S., Canale C., Diaspro A.
Correlative nanoscopy: A multimodal approach to molecular resolution
Microscopy Research and Technique, vol. 84, (no. 10), pp. 2472-2482
2020
Bianchini P., Saggau P., Saggau P., Diaspro A., Diaspro A.
An inertia-free beam scanning device for single-wavelength 2PE-STED nanoscopy
Journal of Physics D: Applied Physics, vol. 53, (no. 32)
2020
Deguchi T., Bianchini P., Palazzolo G., Oneto M., Diaspro A., Duocastella M.
Volumetric Lissajous confocal microscopy with tunable spatiotemporal resolution
Biomedical Optics Express, vol. 11, (no. 11), pp. 6293-6310
2019
Cosentino M., Canale C., Bianchini P., Diaspro A.
AFM-STED correlative nanoscopy reveals a dark side in fluorescence microscopy imaging
Science advances, vol. 5, (no. 6)
All Publications
2024
Kariman B.S., Diaspro A., Bianchini P.
Numerical study of transient absorption saturation in single-layer graphene for optical nanoscopy applications
Scientific Reports, vol. 14, (no. 1)
Article
Journal
2024
Zeaiter L., Dabbous A., Baldini F., Pagano A., Bianchini P., Vergani L., Diaspro A.
Unveiling nuclear chromatin distribution using IsoConcentraChromJ: A flourescence imaging plugin for IsoRegional and IsoVolumetric based ratios analysis
PLoS ONE, vol. 19, (no. 7 July)
Article
Journal
2023
Cuneo L., Castello M., Piazza S., Nepita I., Bianchini P., Vicidomini G., Diaspro A.
A deep learning method to separate fluorophores based on their fluorescence lifetime
Biophysical Journal, vol. 122, (no. 3), pp. 462a-463a
2023
Sheppard C.J.R., Castello M., Tortarolo G., Zunino A., Slenders E., Bianchini P., Vicidomini G., Diaspro A.
Background Rejection in Two-Photon Fluorescence Image Scanning Microscopy
Photonics, vol. 10, (no. 5)
2023
Zeinab Zeaiter L., Baldini F., Cuneo L., Diab F., Bianchini P., Portincasa P., Vergani L., Diaspro A.
Deciphering the chromatin organization and epigenomics involved in adipocyte differentiation and hypertrophy by multimodal nanoscopy
Biophysical Journal, vol. 122, (no. 3S1)
Editorships
2020-2020
Bianchini P.
Frontiers in Physics
2018-2023
Bianchini P.
European Biophysics Journal
2018
Bianchini P.
Microscopy Research and Technique
2014
Bianchini P.
Frontiers in Bioengineering and Biotechnology, Nanobiotechnology
Organized Events
2024
Diaspro A., Bianchini P., Engelbrecht L.
Correlative Multimodal Imaging Workshop
2024
Diaspro A., Bianchini P., Gerritsen H.
Focus on Microscopy 2024
2023
Diaspro A., Bianchini P.
8th NIC@IIT Advanced Microscopy practical workshop
2022
Diaspro A., Bianchini P.
7th NIC@IIT Advanced Microscopy practical workshop
2022
Diaspro A., Sheppard C.J.R., Bianchini P.
MULTIMODAL AND NANOSCALE OPTICAL MICROSCOPY
